Enterococci

Enterococci

All faecal streptococci that grow at pH 9.6, 10° and 45°C and in 6.5% NaCl. Nearly all are members of the genus Enterococcus, and also fulfill the following criteria: resistance to 60°C for 30 min and ability to reduce 0.1% methylene blue. The enterococci are a subset of Faecal Streptococci that grow under the conditions outlined above. Alternatively, enterococci can be directly identified as microorganisms capable of aerobic growth at 44±0.5°C and of hydrolysing 4-methlumbelliferyl-β-D-glucoside (MUD, detecting β-glucosidase activity by blue florescence at 366nm), in the presence of thallium acetate, nalidixic acid and 2,3,5-triphenyltetrazolium chloride (TTC, which is reduced to the red formazan) in the specified medium.

Abbreviations: 
EC
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